…I can’t believe nobody has used “YMMV” so far in this thread..!
The above analysis about not having an O2 sensor is surely correct for any given exact throttle position. To go further, however, any vehicle requires a specific level of power to execute a specific duty cycle regardless of the level of chemical energy in the fuel. So if you are going at a constant 60 mph on an exactly level highway, all the many factors affecting power required being constant, X amount of power will be required. Using gasoline, a specific throttle position will deliver the exact amount of fuel to generate that power. But if the fuel contains 3% less power per unit volume, then the throttle will have to be advanced ever so slightly to inject 3% more fuel to make up the difference. So in theory, regardless of the type of fuel delivery system, even carburetors, the fuel volume used will be greater.
That said, the difference of 3 – 4% energy between gasoline and E10 is hard to observe IMO, given that the many other variables that affect fuel mileage are in total much larger than the slight fuel difference. So although it clearly is a small negative influence on mileage, I think it is rather hard to observe it, despite energetic anecdotal evidence to the contrary…